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apyrase  (New England Biolabs)


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    Structured Review

    New England Biolabs apyrase
    Apyrase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 565 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/apyrase/Apyrase/pm42119982-180-30-32
    Average 97 stars, based on 565 article reviews
    apyrase - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Incubation:

    Article Title: Histone H2B-associated proteins: the Arabidopsis nucleolin 1 binds H2B and facilitates nucleosome disassembly via RNA-dependent mechanism.
    Article Snippet: .. To evaluate whether nucleosome disassembly by AtNuc-L1 requires ATP, Flag-TRAP– immobilized AtNuc-L1 was incubated with S1 + S2 nucleosomes and nuclear extract pretreated with 0.5, 1, or 2 units of apyrase ((#M0398S, New England Biolabs, Ipswich, MA, USA) for 30 min at room temperature to deplete endogenous ATP. ..

    Article Title: Functional details of the integral membrane metallo-protease FtsH revealed by solution NMR spectroscopy
    Article Snippet: .. After the size-exclusion step, hexFtsH was incubated with 2 units (U) of apyrase (New England Biolabs) and 5 mM CaCl 2 , in buffer D, overnight at room temperature (∼21 °C) to ensure complete digestion of any remaining endogenously bound nucleotides. .. The apyrase treated samples were then re-purified on a Superdex 200 Increase column (GE Healthcare) equilibrated with buffer D to remove apyrase from hexFtsH.

    Article Title: Molecular basis of CXC chemokine receptor 3 ligand multispecificity
    Article Snippet: .. After incubating at room temperature for 1 hour, the reaction mixture was added with apyrase (NEB) and incubated overnight at 4°C. ..

    Article Title: Structural insights into coffee bitter taste perception by TAS2R43 receptor
    Article Snippet: .. Then, 50 mU per ml Apyrase (New England Biolabs) and 2 μg ml −1 scFv16 were added and incubated for 30 min at room temperature. .. After collecting membrane fractions by ultracentrifugation at 72,500 g for 30 min (Ti45 rotor, Beckman), the pellets were resuspended in the same lysis buffer in addition with 2 μg ml −1 of scFv16 and 2.5 mU per ml Apyrase (New England Biolabs).

    Article Title: Structural insights into coffee bitter taste perception by TAS2R43 receptor.
    Article Snippet: .. Then, 50 mU per ml Apyrase (New England Biolabs) and 2 μg ml−1 scFv16 were added and incubated for 30 min at room temperature. .. After collecting membrane fractions by ultracentrifugation at 72,500g for 30 min (Ti45 rotor, Beckman), the pellets were resuspended in the same lysis buffer in addition with 2 μg ml−1 of scFv16 and 2.5 mU per ml Apyrase (New England Biolabs).

    other:

    Article Title: Myosin forces remodel F-actin for mechanosensitive protein recognition.
    Article Snippet: Each well was treated with the following sequence: incubation with 500 nM of streptavidin (Rockland) in MB supplemented to 3 mM DTT for 4 min, blocking with Blocking Buffer (MB-PVP supplemented with 1 mg ml−1 κ-casein (Sigma) and 1 mg ml−1 bovine serum albumin (BSA, Gemini)) for 1 min, incubation with capped F-actin extended from biotinylated seeds for 2 min, washing with MB, then immersion in MB + oxygen scavenger mixture (described above) for TIRF imaging.

    Article Title: Cysteine availability tunes ubiquitin signaling via inverse stability of LRRC58 E3 ligase and its substrate CDO1.
    Article Snippet: Each sgRNA was cloned into the pSpCas9 vector using 100ng pSpCas9 vector, 2 μL diluted oligos, 2 μL 10x TANGO buffer (Thermo Fisher Scientific), 1 μL 10mM DTT, 1 μL 10mM ATP, 1 μL Bbs1 (Thermo Fisher Scientific), 0.5 μL T4 Ligase (NEB), and 20 μL dH2O.

    Lysis:

    Article Title: Library docking for Cannabinoid-2 Receptor ligands
    Article Snippet: .. Frozen cell pellets were thawed and resuspended in lysis buffer containing 20 mM HEPES (pH 7.5), 100 mM NaCl, 10 mM MgCl2, 100 μM TCEP, 25 mU/mL apyrase (New England Biolabs), 10 μM CB2 agonist, and protease inhibitors (1 mM benzamidine, 100 μg/mL leupeptin, 2 μg/mL aprotinin, and 2 μg/mL pepstatin). ..



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